Type f botulinum toxin and use thereof

ABSTRACT

The present invention relates to a polypeptide free of toxin activity which gives protection against botulinum type F toxin. The invention also relates to a fusion protein comprising a fragment of a toxin molecule and a purification moiety which enables purification of the fragment from solution. The invention also relates to pharmaceutical compositions comprising the polypeptide or the fusion protein, vaccines comprising the polypeptide, methods of producing the present polypeptides, vaccines and pharmaceutical compositions, and methods of vaccinating a mammal against a botulinum toxin.

[0001] The present invention relates to type F botulinum toxin, to a fragment of type F botulinum neurotoxin, to production of the fragment by recombinant means and to a synthetic gene encoding the fragment. In particular, the invention relates to a novel polypeptide fragment capable of eliciting an immunological response that is protective against type F botulinum neurotoxin (BoNT/F) in man or animals and to a vaccine containing the fragment.

[0002] Botulinum neurotoxins (BoNTs) are high molecular weight proteins (approx. 150,000 Da) which exert potent neuroparalytic effects on vertebrates. They are elaborated by anaerobic Gram-positive bacteria belonging to the genus Clostridium. The majority of clostridia which produce BoNT are classified as Clostridium botulinum. In recent years, however, isolates which resemble Clostridium barati and Clostridium butyricum have been shown to produce BoNT. On the basis of antigenicity, BONT has been subdivided into seven distinct types, designated A to G. All seven neurotoxins (BoNT/A to BoNT/G) are synthesised as a single-chain 150,000 Da molecule which subsequently become nicked to the more potent di-chain form, composed of a heavy (H) chain (approx. 100,000 Da) and a light (L) chain (approx. 50,000 Da) linked by at. least one disulphide bridge.

[0003] The action of BONT involves three distinct phases. In the first phase the toxins become bound to acceptors on the external surface of the targeted neural cells. This is followed by an energy dependent internalisation step in which the toxin, or part of it, enters the cell. Thereafter, the active moiety of the toxin causes nerve cell dysfunction by blocking the intracellular release of the neurotransmitter, acetylcholine, at the nerve periphery, causing flaccid paralysis. The L chain possesses the catalytic activity responsible for cell poisoning and the H chain delivers this moiety to the cell cytoplasm by mediating binding of the toxin to the cell and subsequent internalisation.

[0004] The entire amino acid sequences of all 7 BoNTs are now known (Minton, N. P. (1995). Current Topics in Microbiology and Immunology 195: 161-187), revealing them to be surprisingly divergent in their primary amino acid sequences. Thus, sequence identity amongst the different serotypes generally does not exceed 40%, with those areas of homology localised to discrete domains which are interspersed with amino acid tracts exhibiting little overall similarity. Between the different L chains (average size 439), 63 amino acids are absolutely conserved. Throughout the H chains (average size 843) 97 amino acids are identical. The most notable areas of conservation include: the two cysteine residues involved in the disulphide bond formation between the L and H chain; the histidine rich motif within the L chain associated with metalloprotease activity; and a highly conserved PYI/VXALN-motif found adjacent to regions identified as possessing membrane spanning potential. The most notable tract of sequence divergence amongst toxins is localised to the COOH-terminus of their respective H chains (amino acid 1124 onwards of BoNT/A). This would appear to be consistent with the notion that this domain is involved in neurotoxin binding and that different toxins target different acceptors on neural cell surfaces.

[0005] The effectiveness of modem food-preserving processes in Western countries has made outbreaks of botulism extremely rare. The frequent use of C.botulinum as a test organism in the food industry, and the growing use of the toxin by neurobiochemists, has, however, increased the need for human vaccines. The formulation of these vaccines has changed little since the early 1950s: partially purified preparations of the neurotoxins are toxoided by formaldehyde treatment and absorbed onto precipitated aluminium salts. Using such methodology, polyvalent vaccines (against ABCDE or ABEF) for human immunisation are currently available. Such vaccines suffer from the drawback of low immune response and considerable batch to batch variation due to the high proportion (60-90%) of contaminating proteins in toxoid preparations. Recent work has therefore concentrated on the development of procedures for the purification of toxins to near-homogeneity. The use of purified toxins in the production of vaccines, however, suffers from the drawbacks, first, of having to produce them under high containment and, secondly, of requiring the presence of low levels of formaldehyde to prevent possible reversion-of the toxoid to the active state.

[0006] Production of subunit vaccines against other organisms and toxins has been investigated by a number of laboratories. This work has focused on the best known toxin subtypes, namely A and B, leading to new vaccines giving specific immunity against toxins of type A or B. Each new vaccine, however, may not give protection against other toxin subtypes.

[0007] Recombinant production of vaccine components has brought great advances in vaccine purity and volume of production. A. J. Makoff et al, in Bio/Technology, volume Oct. 7, 1989, pages 1043-1046, describe the expression of a tetanus toxin fragment in E.coli, and its purification and potential use as a vaccine. The technique described nevertheless requires a large number of steps to recover purified vaccine components from the host cells.

[0008] It is an object of this invention to produce a vaccine against a type F botulinum toxin. It is another object to simplify vaccine manufacture. A further object is to improve production of botulinum toxin vaccines. A still further object of the invention is to overcome or at least mitigate problems and/or limitations in existing vaccines and methods of production.

[0009] According to a first aspect of the invention there is provided a polypeptide free of botulinum toxin activity which induces protective immunity to a type F botulinum toxin. The polypeptide is useful in manufacture of a vaccine against type F toxin, and in contrast to prior art compositions such as polyvalent vaccines is not a toxoid and does not need pretreatment with formaldehyde. Also in contrast to prior art compositions the polypeptide is generally of smaller size than the toxin itself.

[0010] An embodiment of the first aspect of the invention provides a polypeptide characterized in that it:

[0011] (a) is free of botulinum toxin activity, and

[0012] (b) is capable of eliciting, in a mammal, an immunological response that is protective against type F botulinum toxin.

[0013] The term “protective” used in conjunction with “immunity” and “immunological response” is used to indicate an increased ability to survive challenge by active botulinum toxin F. This increase is typically mediated by an increased titre of antibodies to the toxin or an increased ability to produce antibodies to the toxin upon challenge with toxin. The term is not intended to indicate absolute protection against any amount of toxin.

[0014] The invention thus offers specific protection against a type F botulinum toxin, protection that has hitherto been unavailable.

[0015] In a particular embodiment the present invention provides a peptide or peptide conjugate comprising the amino acid sequence of the C. botulinum strain Langeland BoNT/F from amino acids 848 to 1278 (SEQ ID NO:1), but lacking the amino acid sequences of the L chain and H_(N) epitopes necessary for metalloprotease activity and toxin internalisation (found between amino acids 1 to 439 and 440 to 847, respectively); the peptide is capable of inducing an immune response that is protective against BoNT/F when administered to humans or other animals.

[0016] In a more particular embodiment the peptides of the invention consist of substantially only the sequence of amino acids from 848 to 1278 (SEQ ID NO:1) of the amino acid sequence of BoNT/F of the Clostridium botulinum strain Langeland, or of that sequence in the form of a fusion peptide with another amino acid sequence not being amino acids 1 to 847 of BoNT/F. The term ‘other amino acid sequence’ will be understood by a person skilled in the art to include complete proteins as well as relatively short amino acid sequences as appropriate to the needs of the user. Optionally, the other amino acid sequence is a non-C. botulinum, antigenic protein which is included fused to the aforesaid sequence for the purpose of providing other immunity or labelling, or for modifying expression of the polypeptide in a host cell.

[0017] In another embodiment of the invention the polypeptide comprises a fragment or a derivative of a type F botulinum neurotoxin free of botulinum toxin activity and capable of induce protective immunity against type F toxin. The fragment is free of toxoid and free of formaldehyde and has a length of less than 700 amino acids, preferably less than 500 amino acids.

[0018] In further specific embodiments of the invention the fragment is selected from:

[0019] (a) amino acids 848-1278 of a type F botulinum toxin, (SEQ ID NO:1)

[0020] (b) amino acids 848-991 of a type F botulinum toxin, (SEQ ID NO:2)

[0021] (c) amino acids 992-1135 of a type F botulinum toxin, (SEQ ID NO:3) and

[0022] (d) amino acids 1136-1278 of a type F botulinum toxin (SEQ ID NO:4).

[0023] The invention also relates to a toxin derivative, being a synthetic polypeptide comprising a plurality of fragments of a type F botulinum toxin linked together in repeated sections. The derivative can-comprise a dimer of the fragments specified above.

[0024] The first aspect of the invention also provides polypeptide compositions, free of botulinum toxin activity and capable of inducing protective immunity against botulinum toxin, which compositions are adapted so as to facilitate their processing. This is of advantage in the manufacture of vaccines as polypeptide must be separated out from a mixture of any components that are undesirable in an eventual vaccine. Such an adapted composition comprises.

[0025] (1) a polypeptide, free of botulinum toxin activity and capable of inducing protective immunity against a botulinum toxin; and

[0026] (2) a polypeptide adapted for purification of the composition.

[0027] Component (2) is adapted, for example, to facilitate purification of the composition from aqueous solution and optionally comprises an antibody, a binding region of an antibody, a polypeptide adapted to bind to an ion exchange column, a polypeptide adapted to bind to an affinity chromatography column or a purification ligand.

[0028] The composition preferably comprises or consists of a single polypeptide including components (1) and (2), for example in the form of a fusion polypeptide.

[0029] In use of the compositions, extraction of the compositions from a mixture such as the supernatant from lysed cells expressing the composition is rendered a simple and fast process. It is particularly advantageous that in the composition, the vaccinating properties of component (1) are substantially retained, meaning that after purification of the composition it is used in a vaccine without the need for further modification, in particular without the need to remove component (2). As candidates for component (1) of the composition, all polypeptides previously described according to the first aspect of the invention are suitable. Further, fragments of tetanus toxin, free of toxin activity, are suitable.

[0030] A polypeptide according to a specific embodiment of the invention thus comprises a fusion protein of:

[0031] (a) amino acids 848 to 1278 (SEQ ID NO:1) of a type F botulinum neurotoxin, with

[0032] (b) a purification moiety.

[0033] It is preferred that the purification moiety is adapted to bind to an affinity chromatography column. A typical purification moiety comprises from 50 to 500 amino acids. In a specific embodiment the fusion protein comprises malaise-binding protein as the purification moiety. This fusion protein is particularly suitable for purification using an affinity chromatography column and has been found to have useful vaccinating properties, as described below.

[0034] According to a second aspect the invention provides a vaccine against a botulinum toxin, comprising a polypeptide of the first aspect of the invention and a pharmaceutically acceptable carrier.

[0035] Suitable carriers are known to a person of skill in the for preparation of the vaccine. In an embodiment hereinafter described the carrier includes Freund's adjuvant. Another suitable carrier component is precipitated alum salts.

[0036] In a third aspect of the present invention there is. provided a recombinant DNA encoding polypeptides of the invention. Such recombinant DNA is conveniently provided by PCR amplification of the DNA coding for the desired sequence, eg., BoNT/F₈₄₈₋₁₂₇₈, using primers targeted at respective ends of the double stranded sequence. Optionally the template sequence used in PCR represents the natural clostridial gene. In a preferred embodiment of the invention, however, the sequence used is a synthetic sequence encoding the same amino acids as the natural clostridial protein but in which codon usage has been altered. It is preferred that the synthetic gene has a GC content of at least 40%, preferably at least 45% and most preferably at least 50%.

[0037] In the case of such a synthetic sequence, insertion into the chosen expression plasmid is achieved, in one embodiment of the invention, through the use of incorporated appropriate restriction endonuclease recognition sites positioned at the extremities of the DNA fragment during its construction.

[0038] By whatever means the recombinant DNA encoding the BoNT/F peptide is generated, it is ligated into a suitable expression vector at which stage genetic fusion to a desired fusion peptide encoding sequence occurs, if desired, and the resultant vector is introduced into a suitable cell line, eg., E. coli or a yeast such as Pichia pastoris. A cell line producing the desired product is selected through established procedures, eg., Western Blotting, or ELISA.

[0039] Fourth and fifth aspects of the invention provide respectively, a plasmid vector incorporating the DNA of the third aspect and a cell line comprising the plasmid and expressing the DNA.

[0040] The invention also provides a method for production of a toxin vaccine in which purification of active vaccinating agent is facilitated by its expression in combination with a polypeptide sequence adapted for purification. Accordingly, a sixth aspect of the invention provides a method for production of a toxin vaccine, said vaccine comprising a vaccinating polypeptide free of toxin activity and capable of inducing protective immunity against a toxin, wherein the method comprises expressing in a host cell a DNA sequence coding for a fusion protein, said fusion protein comprising said vaccinating polypeptide and a purification moiety, obtaining an extract from the host cell comprising the fusion protein, and purifying therefrom the fusion protein.

[0041] In preferred embodiment of the sixth aspect of the invention there is provided a method of producing a vaccine containing a polypeptide of the first aspect of the invention, comprising the steps of:

[0042] (a) expressing in a host cell a DNA encoding a fusion protein, said protein being a fusion of (i) a fragment of a botulinum toxin, said fragment being free of toxin activity and capable of inducing protective immunity against botulinum toxin, and (ii) a purification moiety adapted to bind to an affinity chromatography column,

[0043] (b) obtaining from said host cell an extract comprising the fusion protein, and

[0044] (c) purifying the fusion protein using an affinity chromatography column.

[0045] In use of an embodiment of the invention the fusion protein is removed from the column by elution with a substrate. The method optionally includes cleaving the fusion protein and retaining the toxin fragment. The method has been used specifically with type F toxin but applies also to all other botulinum toxins and to tetanus toxin.

[0046] By this method the invention enables a preparation of botulinum toxin type F fragment free of contamination by other clostridial proteins, these latter frequently contaminating prior art preparations derived from cultures of Clostridium bacteria.

[0047] The fusion protein or toxin fragment obtained is typically in a substantially pure form and suitable for incorporation into a vaccine or other pharmaceutical composition in a few simple steps.

[0048] It should be noted that the creation of certain fusion proteins comprising the BoNT/F-derived peptide is useful in the initial isolation BoNT/F, following which cleavage is optionally employed to purify the BoNT/F-related peptide. Where codons are added at the 5′-end of the BoNT/F-encoding DNA to aid in translation, these amino acids are optionally retained at the NH₂-terminal end of the final peptide, eg., those used to bring about secretion of the peptide or more simply the addition of an NH₂-terminal methionine to initiate translation.

[0049] A seventh aspect of the invention provides a method of making a pharmaceutical composition comprising:

[0050] (a) expressing in a host cell a DNA encoding a fusion protein, said protein being a fusion of (i) a botulinum toxin or a fragment thereof, free of toxin activity and capable of inducing protective immunity against botulinum toxin, and (ii)a purification moiety adapted to bind to an affinity chromatography column,

[0051] (b) obtaining from said host cell an extract comprising the fusion protein,

[0052] (c) purifying the fusion protein using an affinity chromatography column,

[0053] (d) incorporating the purified fusion protein into a pharmaceutical composition.

[0054] The purification moiety typically comprises 50 to 500 amino acids, is water soluble and binds to an affinity chromatography column.

[0055] The inventors have found that a fusion protein retaining the purification moiety is of advantage in producing a vaccine against a type F botulinum toxin. Vaccinating activity is found in the fusion protein, so the purification protein does not need to be removed prior to vaccine manufacture, thus simplifying the manufacturing process. It is preferred that the purification protein is a globular, water soluble protein that binds to and can be purified using an affinity chromatography column. It is further preferred that the purification protein is highly immunogenic. Thus, a particularly preferred fusion protein comprises a fragment of a botulinum toxin free of toxin activity, an immunogenic region and a purification region adapted to bind to an affinity chromatography column.

[0056] The term immunogenic region is used above to describe a sequence of amino acids in a protein that is known to elicit stimulation of the immune system in humans or other animals. Examples of such an immunogenic region include keyhole limpet haemocyanin.

[0057] Further aspects of the invention provide a pharmaceutical containing the fusion protein, methods of vaccinating mammals using the vaccines and compositions of the invention and antisera raised against the polypeptides, vaccines and compositions of the invention.

[0058] There now follows description of specific embodiments of the invention, illustrated by drawings in which:

[0059]FIG. 1: shows the three major domains of a BoNT toxin. The numbers refer to the positions of the amino acids flanking these three domains in BoNT/F of C.botulinum strain Langeland;

[0060]FIG. 2: shows a schematic representation of how synthetic gene blocks were assembled by PCR;

[0061]FIG. 3: shows an example of a recombinant plasmid (pFHC206) made in which the synthetic DNA fragment in FIG. 5 is inserted into the expression plasmid pMal-C2; and

[0062]FIG. 4: shows antibody titres against BoNT/F obtained in mice immunised with MBP-BoNT/F₈₄₈₋₁₂₇₈ recombinant protein.

[0063] SEQ ID NO:5 shows the nucleotide sequence of the region of the BoNT/F gene from Clostridium botulinum type F strain Langeland encoding the H_(C)fragment; SEQ ID NO:6 shows a synthetic DNA sequence encoding the BoNT/F H_(C) fragment which uses codons which are used most frequently in highly expressed genes of E. coli. The codon corresponding to BoNT/F Ser₈₄₈ begins at nucleotide position 12. It is proceeded by a codon specifying a NH₂-terminal methionine codon and restriction sites for Ndel and BamHi. The codon for Asn₁₂₇₈ begins at nucleotide position 1302, and is followed by a translational stop codon (nt 1305-1308) and a restriction site for Xbal;

EXAMPLES

[0064] Generation of a synthetic DNA fragment encoding H_(C) of BoNT/F which makes use of codons utilised by highly expressed E. coli genes

[0065] A synthetic sequence encoding BoNT/F₈₄₈₋₁₂₇₈ was designed by reverse translation of the BoNT/F amino acid sequence using the REVTRANS programme of DNASTAR Inc (Madison, USA). The codon code used was the “strongly expressed E. coli backtranslation code” (SECOLI.RTC). To facilitate the construction, a number of changes were then made to introduce restriction enzyme recognition sites at strategic points along the length of the fragment, including unique flanking proximal sites for BamHl and Ndel a distal flanking site for Xbal and internal sites for Hpal, Mlul and Sp/l. The gene was then constructed from overlapping 100 mer oligonucleotides by a procedure essentially as described elsewhere [Sandhu et al (1992) Biotechniques 12:14-16].

[0066] Briefly, the gene was constructed as 4 individual blocks (A, B, C and D), each of approximately 350 bp in size. Each block was assembled from 4×100 mer alternating oligonucleotides which overlapped with each other by 20 nucleotides. These 4 oligonucleotides were used in a PCR to generate a composite c.350 bp double-stranded DNA fragment, which was subsequently amplified using 20 mer flanking primers. The amplified fragments of each block were then cloned directly into plasmid pCRll (invitrogen Corp). The flanking primers of all 4 blocks were designed to include restriction enzyme sites which would allow their subsequent assembly into B by Hpal (5′) and Mlul (3′), block C by Mlul (5′) and Sp1L (3′), and block D by Sp1l (5′) and Xbal (3′). Each block was, therefore, released from their respective pCRll-derived recombinant plasmid by cleavage with the appropriate enzyme and the isolated fragments ligated to pMTL23 [Chambers et al (1988). Gene 68:139-149] plasmid DNA which had been cleaved with BamHI and Xbal. A clone was then selected in which all 4 blocks had been inserted in the expected order. This was confirmed by nucleotide sequencing using routine methods [Maniatis et al. (1989). Molecular Cloning a Laboratory Manual. Cold Spring Harbor Laboratory Press], and the plasmid obtained designed pFHC23.

[0067] Generation of a H_(C) peptide (848 to 1278) of BoNT/F of C. botulinum strain Langeland

[0068] A candidate vaccine against the BoNT/F of C. botulinum was produced by expressing the fragment of the synthetic gene encoding the H_(C) fragment, amino acids 848 to 1278. This DNA fragment was isolated from plasmid pFHC23 as an approximately 1.3 kb BamHl-Xhol restriction fragment and inserted between the unique BamHil and SaA sites of pUC9 [Vieira and Messing (1982). Gene 19: 259-268], generating the plasmid pFHC29. -The insert was then reisolated from pFHC29 as an EcoRI-Xbal fragment and inserted between the equivalent sites of the commercially available expression vector pMal-c2 (New England Biolabs), to yield the final plasmid pFHC206. The resultant plasmid expressed BoNT/F₈₄₈₋₁₂₇₈ as a fusion protein with the vector encoded malaise binding protein (MBP).

[0069] Fusion protein product (MBP-BoNT/F₈₄₈₋₁₂₇₈) was prepared from the cell line containing pFHC206 in the following manner. E. coli containing pFHC206 was cultivated in 1 liter of media (M9, supplemented with 0.8M sorbitol, 0.5% casamino acids, 50μg/ml ampicillin), shaking (200 rpm) at 37° C. until an OD₆₀₀ of 1.0 was achieved. At this point IPTG was added at a final concentration of 1 mM and shaking continued at 27° C. for a further 4 hour. Cells were harvested by centrifugation (5000×g) and resuspended in 20 ml of lysis buffer (Protein Fusion and Purification System, New England Biolabs) and cells disrupted by sonication. Lysate was applied to a GPC column containing 180 ml of Sephacel S100, and the protein in the void fraction collected. MBP-BoNT/F H₈₄₈₋₁₂₇₈ fusion protein in this fraction was then allowed to adsorb at room temperature to a 4-6 ml volume of Amylose resin (New England Laboratories) over a 3 hour period with gentle shaking (10 rpm). Recombinant fusion protein was then eluted in buffer (0.01 M Tris HCl, pH 7.0) containing 5 mM malaise. Eluted protein was concentrated using an Amicon PM30 membrane filter.

[0070] Toxicity of candidate vaccine

[0071] The toxicity of the candidate vaccine fusion peptide was determined by intraperitoneal inoculation of 25 μg amounts of the total recombinant MBP-BoNT/F₈₄₈₋₁₂₇₈ protein into groups of 4 mice. The candidate vaccine was well tolerated and mice showed no signs of acute or chronic toxicity up to 2 weeks post inoculation.

[0072] Antibody responses to candidate vaccines

[0073] The candidate vaccine was administered to groups of 4 mice by intraperitoneal inoculation in complete Freund's adjuvant, and a booster inoculation given on 3 further occasions at two week intervals. Antibody response against purified C. botulinum strain Langeland BoNT/F was monitored by ELISA (FIG. 4).

[0074] Protection against toxin challenge

[0075] Animals which were immunised with MBP-BoNT/F₈₄₈₋₁₂₇₈ fusion protein were subjected to an intraperitoneal challenge with various doses of purified C. botulinum strain Langeland BoNT/F. At doses of 12 LD₅₀ and above, all the control, unimmunised mice succumbed within 24 hour. All immunised groups of mice survived challenges of up to 2.4×10⁴ LD₅₀ . One of the immunised mice which had survived an initial challenge of 1.8, LD₅₀ was subsequently shown to be immune to a further challenge of 10⁶ LD₅₀. TABLE 1 Protection against challenge with C. botulinum strain Langeland BoNT/F afforded by the MBP-BoNT/F₈₄₈₋₁₂₇₈ fusion protein vaccine. A total of 4 × 25 μg intraperitoneal doses of antigen mixed with adjuvant were given to groups of 4 mice at 14 day intervals. After 50 days mice were subjected to intraperitoneal challenges of varying levels of purified BoNT/F, (isolated from C. botulinum strain Langeland), and deaths recorded up to 4 days. Mortality/Total Animals Challenge Dose (LD₅₀) Control Animals Immunised Animals 2.4 × 10⁴ 4/4 0/4 3.6 × 10³ 4/4 0/4 5.4 × 10² 4/4 0/4 81 4/4 0/4 12 4/4 0/4   1.8 2/4  0/4^(a)

[0076] This invention provides a fragment (such as amino acids 848-1278) of BoNT/F isolated from C. botulinum strain Langeland for use as a vaccine. The fragment retains its immunogenic properties while still fused with MBP, dispensing with the need for an additional purification step. The recombinant fusion protein appears to be non-toxic in mice at doses up to 25 μg. Repeated doses produced a significant antibody response which protects animals against BoNT/F challenge. As a vaccine it offers several advantages over neurotoxin toxoided by formaldehyde treatment. Most notably, it may be prepared more easily and, due to the absence of active toxin, at a lower level of containment. The absence of other contaminating C.botulinum proteins and partially toxoided materials also make it inherently safer for vaccine application and less reactogenic.

1 6 431 amino acids amino acid linear peptide 1 Ser Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr 1 5 10 15 Lys Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn 20 25 30 Lys Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly 35 40 45 Asp Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser 50 55 60 Ser Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr 65 70 75 80 Asn Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro 85 90 95 Lys Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp 100 105 110 Cys Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn 115 120 125 Lys Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu 130 135 140 Val Phe Asn Tyr Thr Gln Met Ile Ser Ile Ser Asp Tyr Ile Asn Lys 145 150 155 160 Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Gly Asn Ser Arg Ile 165 170 175 Tyr Ile Asn Gly Asn Leu Ile Asp Glu Lys Ser Ile Ser Asn Leu Gly 180 185 190 Asp Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile Val Gly Cys Asn 195 200 205 Asp Thr Arg Tyr Val Gly Ile Arg Tyr Phe Lys Val Phe Asp Thr Glu 210 215 220 Leu Gly Lys Thr Glu Ile Glu Thr Leu Tyr Ser Asp Glu Pro Asp Pro 225 230 235 240 Ser Ile Leu Lys Asp Phe Trp Gly Asn Tyr Leu Leu Tyr Asn Lys Arg 245 250 255 Tyr Tyr Leu Leu Asn Leu Leu Arg Thr Asp Lys Ser Ile Thr Gln Asn 260 265 270 Ser Asn Phe Leu Asn Ile Asn Gln Gln Arg Gly Val Tyr Gln Lys Pro 275 280 285 Asn Ile Phe Ser Asn Thr Arg Leu Tyr Thr Gly Val Glu Val Ile Ile 290 295 300 Arg Lys Asn Gly Ser Thr Asp Ile Ser Asn Thr Asp Asn Phe Val Arg 305 310 315 320 Lys Asn Asp Leu Ala Tyr Ile Asn Val Val Asp Arg Asp Val Glu Tyr 325 330 335 Arg Leu Tyr Ala Asp Ile Ser Ile Ala Lys Pro Glu Lys Ile Ile Lys 340 345 350 Leu Ile Arg Thr Ser Asn Ser Asn Asn Ser Leu Gly Gln Ile Ile Val 355 360 365 Met Asp Ser Ile Gly Asn Asn Cys Thr Met Asn Phe Gln Asn Asn Asn 370 375 380 Gly Gly Asn Ile Gly Leu Leu Gly Phe His Ser Asn Asn Leu Val Ala 385 390 395 400 Ser Ser Trp Tyr Tyr Asn Asn Ile Arg Lys Asn Thr Ser Ser Asn Gly 405 410 415 Cys Phe Trp Ser Phe Ile Ser Lys Glu His Gly Trp Gln Glu Asn 420 425 430 144 amino acids amino acid linear peptide 2 Ser Tyr Thr Asn Asp Lys Ile Leu Ile Leu Tyr Phe Asn Lys Leu Tyr 1 5 10 15 Lys Lys Ile Lys Asp Asn Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn 20 25 30 Lys Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly 35 40 45 Asp Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser 50 55 60 Ser Lys Pro Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr 65 70 75 80 Asn Gly Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro 85 90 95 Lys Tyr Phe Asn Lys Val Asn Leu Asn Asn Glu Tyr Thr Ile Ile Asp 100 105 110 Cys Ile Arg Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Asn Tyr Asn 115 120 125 Lys Ile Ile Trp Thr Leu Gln Asp Thr Ala Gly Asn Asn Gln Lys Leu 130 135 140 144 amino acids amino acid linear peptide 3 Val Phe Asn Tyr Thr Gln Met Ile Ser Ile Ser Asp Tyr Ile Asn Lys 1 5 10 15 Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Gly Asn Ser Arg Ile 20 25 30 Tyr Ile Asn Gly Asn Leu Ile Asp Glu Lys Ser Ile Ser Asn Leu Gly 35 40 45 Asp Ile His Val Ser Asp Asn Ile Leu Phe Lys Ile Val Gly Cys Asn 50 55 60 Asp Thr Arg Tyr Val Gly Ile Arg Tyr Phe Lys Val Phe Asp Thr Glu 65 70 75 80 Leu Gly Lys Thr Glu Ile Glu Thr Leu Tyr Ser Asp Glu Pro Asp Pro 85 90 95 Ser Ile Leu Lys Asp Phe Trp Gly Asn Tyr Leu Leu Tyr Asn Lys Arg 100 105 110 Tyr Tyr Leu Leu Asn Leu Leu Arg Thr Asp Lys Ser Ile Thr Gln Asn 115 120 125 Ser Asn Phe Leu Asn Ile Asn Gln Gln Arg Gly Val Tyr Gln Lys Pro 130 135 140 143 amino acids amino acid linear peptide 4 Asn Ile Phe Ser Asn Thr Arg Leu Tyr Thr Gly Val Glu Val Ile Ile 1 5 10 15 Arg Lys Asn Gly Ser Thr Asp Ile Ser Asn Thr Asp Asn Phe Val Arg 20 25 30 Lys Asn Asp Leu Ala Tyr Ile Asn Val Val Asp Arg Asp Val Glu Tyr 35 40 45 Arg Leu Tyr Ala Asp Ile Ser Ile Ala Lys Pro Glu Lys Ile Ile Lys 50 55 60 Leu Ile Arg Thr Ser Asn Ser Asn Asn Ser Leu Gly Gln Ile Ile Val 65 70 75 80 Met Asp Ser Ile Gly Asn Asn Cys Thr Met Asn Phe Gln Asn Asn Asn 85 90 95 Gly Gly Asn Ile Gly Leu Leu Gly Phe His Ser Asn Asn Leu Val Ala 100 105 110 Ser Ser Trp Tyr Tyr Asn Asn Ile Arg Lys Asn Thr Ser Ser Asn Gly 115 120 125 Cys Phe Trp Ser Phe Ile Ser Lys Glu His Gly Trp Gln Glu Asn 130 135 140 1293 base pairs nucleic acid double linear DNA (genomic) 5 TCATATACTA ATGATAAAAT TCTAATTTTA TATTTTAATA AATTATATAA AAAAATTAAA 60 GATAACTCTA TTTTAGATAT GCGATATGAA AATAATAAAT TTATAGATAT CTCTGGATAT 120 GGTTCAAATA TAAGCATTAA TGGAGATGTA TATATTTATT CAACAAATAG AAATCAATTT 180 GGAATATATA GTAGTAAGCC TAGTGAAGTT AATATAGCTC AAAATAATGA TATTATATAC 240 AATGGTAGAT ATCAAAATTT TAGTATTAGT TTCTGGGTAA GGATTCCTAA ATACTTCAAT 300 AAAGTGAATC TTAATAATGA ATATACTATA ATAGATTGTA TAAGGAATAA TAATTCAGGA 360 TGGAAAATAT CACTTAATTA TAATAAAATA ATTTGGACTT TACAAGATAC TGCTGGAAAT 420 AATCAAAAAC TAGTTTTTAA TTATACACAA ATGATTAGTA TATCTGATTA TATAAATAAA 480 TGGATTTTTG TAACTATTAC TAATAATAGA TTAGGCAATT CTAGAATTTA CATCAATGGA 540 AATTTAATAG ATGAAAAATC AATTTCGAAT TTAGGTGATA TTCATGTTAG TGATAATATA 600 TTATTTAAAA TTGTTGGTTG TAATGATACA AGATATGTTG GTATAAGATA TTTTAAAGTT 660 TTTGATACGG AATTAGGTAA AACAGAAATT GAGACTTTAT ATAGTGATGA GCCAGATCCA 720 AGTATCTTAA AAGACTTTTG GGGAAATTAT TTGTTATATA ATAAAAGATA TTATTTATTG 780 AATTTACTAA GAACAGATAA GTCTATTACT CAGAATTCAA ACTTTCTAAA TATTAATCAA 840 CAAAGAGGTG TTTATCAGAA ACCAAATATT TTTTCCAACA CTAGATTATA TACAGGAGTA 900 GAAGTTATTA TAAGAAAAAA TGGATCTACA GATATATCTA ATACAGATAA TTTTGTTAGA 960 AAAAATGATC TGGCATATAT TAATGTAGTA GATCGTGATG TAGAATATCG GCTATATGCT 1020 GATATATCAA TTGCAAAACC AGAGAAAATA ATAAAATTAA TAAGAACATC TAATTCAAAC 1080 AATAGCTTAG GTCAAATTAT AGTTATGGAT TCAATAGGAA ATAATTGCAC AATGAATTTT 1140 CAAAACAATA ATGGGGGCAA TATAGGATTA CTAGGTTTTC ATTCAAATAA TTTGGTTGCT 1200 AGTAGTTGGT ATTATAACAA TATACGAAAA AATACTAGCA GTAATGGATG CTTTTGGAGT 1260 TTTATTTCTA AAGAGCATGG ATGGCAAGAA AAC 1293 1313 base pairs nucleic acid double linear DNA (genomic) 6 GGATCCATAT GTCTTACACT AACGACAAAA TCCTGATCCT GTACTTCAAC AAACTGTACA 60 AAAAAATCAA AGACAACTCT ATCCTGGACA TGCGTTACGA AAACAACAAA TTCATCGACA 120 TCTCTGGCTA TGGTTCTAAC ATCTCTATCA ACGGTGACGT CTACATCTAC TCTACTAACC 180 GCAACCAGTT CGGTATCTAC TCTTCTAAAC CGTCTGAAGT AAACATCGCT CAGAACAACG 240 ACATCATCTA CAACGGTCGT TACCAGAACT TCTCTATCTC TTTCTGGGTT CGTATCCCGA 300 AATACTTCAA CAAAGTTAAC CTGAACAACG AATACACTAT CATCGACTGC ATCCGTAACA 360 ACAACTCTGG TTGGAAAATC TCTCTGAACT ACAACAAAAT CATCTGGACT CTGCAGGACA 420 CTGCTGGTAA CAACCAGAAA CTGGTTTTCA ACTACACTCA GATGATCTCT ATCTCTGACT 480 ACATTAATAA ATGGATCTTC GTTACTATCA CTAACAACCG TCTGGGTAAC TCTCGTATCT 540 ACATCAACGG TAACCTGATC GATGAAAAAT CTATCTCTAA CCTGGGTGAC ATCCACGTTT 600 CTGACAACAT CCTGTTCAAA ATCGTTGGTT GCAACGACAC GCGTTACGTT GGTATCCGTT 660 ACTTCAAAGT TTTCGACACT GAACTGGGTA AAACTGAAAT CGAAACTCTG TACTCTGACG 720 AACCGGACCC GTCTATCCTG AAAGACTTCT GGGGTAACTA CCTGCTGTAC AACAAACGTT 780 ACTACCTGCT GAACCTGCTC CGGACTGACA AATCTATCAC TCAGAACTCT AACTTCCTGA 840 ACATCAACCA GCAGCGTGGT GTTTATCAGA AACCTAATAT CTTCTCTAAC ACTCGTCTGT 900 ACACTGGTGT TGAAGTTATC ATCCGTAAAA ACGGTTCTAC TGACATCTCT AACACTGACA 960 ACTTCGTACG TAAAAACGAC CTGGCTTACA TCAACGTTGT TGACCGTGAC GTTGAATACC 1020 GTCTGTACGC TGACATCTCT ATCGCTAAAC CGGAAAAAAT CATCAAACTG ATCCGTACTT 1080 CTAACTCTAA CAACTCTCTG GGTCAGATCA TCGTTATGGA CTCGATCGGT AACAACTGCA 1140 CTATGAACTT CCAGAACAAC AACGGTGGTA ACATCGGTCT GCTGGGTTTC CACTCTAACA 1200 ACCTGGTTGC TTCTTCTTGG TACTACAACA ACATCCGTAA AAACACTTCT TCTAACGGTT 1260 GCTTCTGGTC TTTCATCTCT AAAGAACACG GTTGGCAGGA AAACTAATCT AGA 1313 

1. A polypeptide free of botulinum toxin activity and free of toxoid which induces protective immunity to a type F botulinum toxin.
 2. A polypeptide characterized in that it: (a) is free of botulinum toxin activity. (b) is free of toxoid, and (c) is capable of eliciting, in a mammal, an immunological response that is protective against type F botulinum toxin.
 3. A polypeptide according to claim 1 or 2 comprising a fragment or a derivative of a heavy chain of a type F botulinum neurotoxin.
 4. A polypeptide according to claim 3 wherein said fragment or said derivative is up to 600 amino acids long.
 5. A polypeptide according to claims 3 or 4 wherein said fragment is selected from: (a) amino acids 848-1278 of a type F botulinum toxin, (b) amino acids 848-991 of a type F botulinum toxin, (c) amino acids 992-1135 of a type F botulinum toxin, and (d) amino acids 1136-1278 of type F botulinum toxin.
 6. A polypeptide according to claims 3 or 4 wherein said derivative comprises a dimer of the fragment according to any of (a)-(d) of claim
 5. 7. A polypeptide composition for use in manufacture of a vaccine, said composition comprising: (1) a polypeptide free of toxin activity and capable of inducing, in a mammal, protective immunity against a botulinum toxin; and (2) a polypeptide adapted to facilitate or enhance purification of the composition.
 8. A polypeptide composition according to claim 7 wherein the composition comprises a fusion protein of (1) and (2).
 9. A polypeptide composition according to claim 7 or 8 comprising: (1) a polypeptide according to any of claims 1-6; and (2) a polypeptide adapted to bind to a chromatography column.
 10. A polypeptide composition according to any of claims 7-9 comprising a polypeptide adapted to bind to an affinity chromatography column.
 11. A polypeptide according to claim 8 comprising a fusion protein of: (a) amino acids 848 to 1278 of a type F botulinum neurotoxin, with (b) a purification moiety.
 12. A vaccine comprising a pharmaceutically acceptable carrier and a polypeptide according to any of claims 1-6 or a polypeptide composition according to any of claims 7-11.
 13. A recombinant DNA encoding a polypeptide according to any of claims 1-6 or a polypeptide composition according to any of claims 7-11.
 14. A method of producing a polypeptide according to any of claims 1-6 or a polypeptide composition according to any of claims 7-11 comprising the steps of: (a) expressing in a host cell a DNA encoding a fusion protein, said protein being a fusion of (i) a fragment or derivative of a type F botulinum toxin, and (ii) a moiety adapted to bind to a chromatography column, (b) obtaining from said host cell an extract comprising the fusion protein, and (c) purifying the fusion protein using a chromatography column.
 15. A method according to claim 14 wherein the chromatography column is an affinity chromatography column and the fusion protein is removed from the column by elution with a substrate.
 16. A method according to claim 14 or 15 further comprising cleaving the fusion protein and retaining the toxin fragment or derivative.
 17. A method of making a pharmaceutical composition comprising: (a) expressing in a host cell a DNA encoding a fusion protein, said protein being a fusion of (i) a polypeptide free of toxin activity and capable of inducing protective immunity against a botulinum toxin, and (ii) a purification moiety adapted to bind to a chromatography column, (b) obtaining from said host cell an extract comprising the fusion protein, (c) purifying the fusion protein using chromatography column, (d) incorporating the purified fusion protein into a pharmaceutical composition.
 18. A method according to claim 17 wherein said purification moiety binds to an affinity chromatography column.
 19. A pharmaceutical composition comprising: (a) a fusion protein, said protein being a fusion of (i) a polypeptide fee of toxin activity and capable of inducing protective immunity against a botulinum toxin, and (ii) a polypeptide adapted to bind to a chromatography column; and (b) a pharmaceutically acceptable carrier.
 20. A pharmaceutical composition according to claim 19 wherein said fusion protein comprises a polypeptide according to any of claims 1-6.
 21. A pharmaceutical composition according to claim 19 or 20 wherein the fusion protein comprises a polypeptide adapted to bind to an affinity chromatography column.
 22. A method of vaccinating a mammal against a botulinum toxin, comprising administering to said mammal a vaccine according to claim
 12. 23. A method of vaccinating a mammal against a botulinum toxin, comprising administering to said mammal a pharmaceutical composition according to any of claims 19-21. 